Structural transitions in the acid-denatured ficin induced by halogenols and alkanols
نویسندگان
چکیده
The effect of eight different alcohols including halogenols and alkanols on the structural transformation of aciddenatured ficin (ADF) at pH 2.5 was studied using farand near-UV circular dichroism (CD) and tryptophan fluorescence. Except methanol and ethanol, other alcohols induced α-helical structure in ADF, as revealed by the increase in MRE222nm values. The fluoro alcohols tested showed a higher helix-inducing potential compared to 2chloroethanol and alkanols. Their effectiveness followed the order: 1,1,1,3,3,3-hexafluoro-2-propanol > 2,2,2trifluoroethanol > tert-butanol > 2-chloroethanol > 1-propanol > 2-propanol. Near-UV CD spectra showed disruption of the tertiary structure in presence of alcohols. Tryptophan fluorescence of ADF was affected differently in presence of these alcohols, showing quenching with fluoro alcohols and enhancement with 2-chloroethanol and alkanols in the order: tert-butanol > 2-propanol > 1-propanol > ethanol > methanol. The obtained results suggested that the effectiveness of the alcohols correlated well with the number of fluorine atoms, bulkiness and arrangement of different alkyl groups.
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